Anti-RIL抗体[EPR16199]-抗体-抗体-生物在线

Anti-RIL抗体[EPR16199]

产品名称： Anti-RIL抗体[EPR16199]

英文名称： Enigma homolog antibody LIM domain protein antibody LIM domain protein RIL antibody LIM protein RIL antibody PDLI4_HUMAN antibody PDLIM 4 antibody PDL

产品编号： ab197026

产品价格： null

产品产地：英国

品牌商标： abcam

更新时间： 2023-09-21T08:07:31

使用范围：

深圳市宇德立生物科技有限公司

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产品详情

经测试应用WB, Flow Cytmore details
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human RIL aa 50-250. The exact sequence is proprietary.
Database link: P50479

Run BLAST with Run BLAST with
阳性对照
- PC-3, A431 and SH-SY5Y whole cell lysates; SH-SY5Y cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.

Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
浓度100 µl 浓度为 0.749 mg/ml 40 µl 浓度为 0.749 mg/ml 10 µl 浓度为 0.749 mg/ml
纯度Protein A purified
克隆单克隆
克隆编号EPR16199
同种型IgG
研究领域

应用

Our Abpromise guarantee covers the use of ab197026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
WB		1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Flow Cyt		1/140. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

组织特异性Isoform 2 is found in brain.
序列相似性Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
翻译后修饰Phosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13.
Information by UniProt
数据库链接
- Entrez Gene: 8572 Human
- Omim: 603422 Human
- SwissProt: P50479 Human
- Unigene: 424312 Human
别名
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all

Anti-RIL antibody [EPR16199] 图像

Western blot - Anti-RIL antibody [EPR16199] (ab197026)

Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution + PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size : 35 kDa
Observed band size : 35 kDa

Exposure time : 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-RIL antibody [EPR16199] (ab197026)

Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution + A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size : 35 kDa
Observed band size : 35 kDa

Exposure time : 1 minute
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-RIL antibody [EPR16199] (ab197026)

Anti-RIL antibody [EPR16199] (ab197026) at 1/10000 dilution + SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size : 35 kDa
Observed band size : 35 kDa

Exposure time : 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.