这种产品利用的是RNA干扰的方法，所谓RNA干扰是指通过双链RNA分子进入细胞可以选择性地沉默相应基因的表达。针对每个蛋白，我们提供两个一样有效的siRNA（siRNA I 和 II）。另外，荧光标记的非目标fluorescein-labeled non-targeted siRNA control #6201 可用作对照监控转染效率，而非偶联的对照unconjugated control siRNA #6568 可以用来控制特异性。现在有针对人和小鼠目标蛋白的SignalSilence® siRNA。  
�l @�ѕacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; font-family: Verdana, Helvetica, Arial, sans-serif; line-height: 22.1875px;"> 可用作对照监控转染效率，而非偶联的对照unconjugated control siRNA #6568 可以用来控制特异性。现在有针对人和小鼠目标蛋白的SignalSilence^® siRNA。  

RNA interference is a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. SignalSilence® Control siRNA (Fluorescein Conjugate) from Cell Signaling Technology (CST) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is fluorescein-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.

干扰是一种通过向细胞中输入双链RNA分子，选择性沉默基因表达的方法。一种特定的小干扰RNA的引入可以通过降解RNA来阻止目的蛋白的翻译。SignalSilence ® Control siRNA （荧光标记）是一种小干扰RNA（siRNA），不能导致细胞信号的特定降解，而用来作为靶siRNA转染的阴性对照。另外，这种siRNA带荧光标记，可以使实验者通过荧光显微镜评估转染效率。

Fluorescent Detection

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. TransIT-TKO® Transfection Reagent from Mirus was used (Mirus catalog #MIR2114).荧光检测用SignalSilence ® Control siRNA (荧光标记) 转染24小时后的活HeLa细胞，转染效率近100%。 .所用转染试剂为Mirus公司的TransIT-TKO ® Transfection Reagent (Mirus catalog #MIR2114)。

Western Blotting

Western blot analysis of extracts from HeLa cells, either untransfected or transfected with NF-κB p65 siRNA #6261 or SignalSilence® Control siRNA (Fluorescein Conjugate), using NF-κB p65 Antibody #3034.Western blot分析HeLa细胞提取物。未转染组和转染组用NF-κB p65 siRNA #6261 或 SignalSilence ® Control siRNA (荧光表记)，所用抗体为 NF-κB p65 Antibody #3034.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) 24 to 48 hours prior to cell lysis. For transfection procedure, follow protocol provided by transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

建议裂解细胞前用100nM Control siRNA II 转染48-72个小时。请参考转染试剂生产商提供的转染步骤。如有问题，请联系CST公司。

6202 SignalSilence® ILK1 siRNA I

6211 SignalSilence® Akt siRNA I

6231 SignalSilence® p53 siRNA I

6241 SignalSilence® Chk1 siRNA I

6251 SignalSilence® PTEN siRNA I

6261 SignalSilence® NF-κB p65 siRNA I