Product: Anti-Epithelial Keratin Type I, clone AE-1
Cat. No: MC-975 (250 μg)
Background:
This antibody recognizes most of the acidic
(type I) keratins. Each epithelium contains at
least one acidic keratin. AE-1 is broadly reactive
with almost all epithelial and their neoplasms.
The antibody exhibits heterogenous staining
within a given epithelium; the detailed staining
pattern varies depending on the differentiated
and/or growth state of the individual cells. This
antibody together with MC-976 (clone AE-3) has
been used for studying the immuno-localization
of epidermal keratins, tissue distribution and
epithelial development. AE-1 and AE-3 have
also been used extensively, individually or mixed,
for distinguishing carcinoma from non-carcinoma.
Specificity: AE-1 detects in immunoblots
distinct bands of 40 kDa, 45 kDa, 46 kDa, 48
kDa, 50 kDa, (50’ kDa), 51 kDa, 55 kDa, and
56.5 kDa with all Ip’s lower than 5.7. These
MW’s are believed to correspond with keratin 19,
18, 17, 16, 15, 14, 13, 12 and 10, respectively.
Species Reactivity: Human, bovine, chicken,
mouse, rabbit, and rat. Others not tested.
Ig Isotype:
Mouse IgG1
Format:
250 μg purified IgG at 1 mg/ml with 0.1 sodium
azide.
Storage and Stability: Store undiluted
antibody at 2-8oC. For a long-term storage,
aliquot and store at -20oC.
Applications and Suggested Dilutions:

Immunohistochemistry: (Formalin-fixed /
Paraffin-embedded sections) Use at 1:150-
1:200 for 30 min. at 37°C. Higher dilutions
can be used if sections are incubated at 4°C
overnight.

Western blot: Use at 1:100.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product