Product: Anti-BIK pAb
Cat. No.: PC-522 (100 μg)
Synonyms:
Anti-Bcl-2-Interacting Killer, anti-Apoptosis
Inducer NBK.
Background:
Apoptosis plays a major role in normal organism
development, tissue homeostasis, and removal
of damaged cells and is caused by the activation
of proteolytic enzymes termed caspases.
Proteins that comprise the Bcl-2 family appear to
control the activation of these enzymes. One
such protein BIK was recently identified as an
endoplasmic reticulum (ER)-residing proapoptotic
member of the Bcl-2 homology
domain-3 (BH3)-only group of the Bcl-2 family
that stimulates mitochondrial release of
cytochrome c following p53 induction of
apoptosis. A significant fraction of BIK is found
as an ER transmembrane protein, with most of
the protein facing the cytosol. Restricting BIK to
the ER membrane by replacing the
transmembrane region with that of the ERselective
membrane anchor of cytochrome b
resulted in a decreased cytochrome c release
from mitochondria and a corresponding drop in
cell death. Recent evidence suggests that BIK
cooperates with NOXA, another BH3-only
protein, to somehow enhance the activation of
Bax to stimulate the rapid release of cytochrome
c from mitochondria.
Specificity:
Recognizes BIK.
Positive Control:
Jurkat cell lysate.
Species Reactivity:
Human and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
A 15 amino acid peptide from near the aminoterminus
of human BIK (GenBank Accession No.
CAG30276).
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Application and Suggested Dilution:

Western blot: Use at 1-2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure: Western blot analysis of BIK in Jurkat cell
lysate with anti-BIK pAb at (A) 1 μg/mL and (B) 2
μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.