HITRAP IEX SELECTION KIT-色谱柱 -耗材-生物在线
上海基星生物科技有限公司
HITRAP IEX SELECTION KIT

HITRAP IEX SELECTION KIT

商家询价

产品名称: HITRAP IEX SELECTION KIT

英文名称: HITRAP IEX SELECTION KIT

产品编号: 17-6002-33

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

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17-6002-33 HITRAP IEX SELECTION KIT
18-1142-75 REC PROTEIN PURIF HANDBOOK
28-4010-81 HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77 HISTRAP FF CRUDE KIT
28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP, 5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit, 7x1
28-9075-46 StrepTrap HP 5 x 1mL
28-9075-47 StrepTrap HP 1 x 5mL
28-9075-48 StrepTrap HP 5 x 5mL
28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml

HiTrap™ IEX Selection Kit

  • Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1-ml HiTrap™ columns.
  • Excellent for optimization studies: buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
  • Excellent for screening different media: includes seven 1-ml HiTrap™ columns prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), ANX Sepharose™ 4 Fast Flow (high sub) (weak anion), Q Sepharose™ XL (strong anion), and SP Sepharose™ XL (strong cation), connectors, and instructions.
  • Designed for use with syringe, peristaltic pump and other chromatography systems such as ÄKTA™design.

HiTrap™ IEX Selection Kit

Technical Information


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Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.

 

ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

 

Q and SP Sepharose™ XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

 

The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.

 

TECHNICAL SPECIFICATIONS
Media  Q Sepharose™ Fast Flow 
  SP Sepharose™ Fast Flow 
  CM Sepharose™ Fast Flow 
  DEAE Sepharose™ Fast Flow 
  ANX Sepharose™ 4 Fast Flow (high sub) 
  Q Sepharose™ XL 
  SP Sepharose™ XL 
Column volume  1 ml 
Max. flow rate*  4 ml/min 
Recommended flow rate  1 ml/min 
Max. back pressure  3 bar (0.3 MPa, 43 psi) 
Storage  20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) 
Storage temperature  4°C to 30°C 
* H2O at 25°C

 

 

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