Product: Anti-MRP6, clone M6II-7
Cat. No: MC-488 (1 ml)
Background:
M6II-7 reacts with MRP6, a 190-200 kDa
transmembrane protein that is related to the
multidrug resistance related protein MRP.
Mutations in the MRP6 gene are responsible for
the connective tissue disorder Pseudoxanthoma
elasticum (PXE).
Specificity:
M6II-7 did not cross-react with the human MDR1,
MRP1, MRP2, MRP3, MPR4 and MRP5 gene
products.
Species Reactivity:
Human, others not tested.
Ig Isotype:
Rat IgG2a
Immunogen:
M6II-7 was raised against a bacterial fusion
protein of human MRP6, containing amino acids
764-964, spanning the putative 12th
transmembrane region as well as predicted
internal and external regions of the protein.
Hybridoma:
The hybridomas cell line was obtained by fusion
of lymph node cells from an immunized rat
(Wistar) with SP2/O mouse myeloma cells.
Format:
1 ml vial containing antibody in a serum-free
culture supernatant with 1 BSA and 0.1
sodium azide. The concentration is
approximately 250 μg/ml. The culture medium is
RPMI-1640 supplemented with Nutridoma-SR.
The medium does not contain serum or added
enzymes. The antibody solution has been
filtered through a 0.22 micron filter.
Storage and Stability:
Store at 2 - 8°C short term. For long term, store
at -20°C.
Applications and Suggested Dilutions:

Immunocytochemistry: Use at approximately
1:20–50 dilution on acetone fixed cytospin
preparations.

Western blotting: Use at approximately 1:20-
50 dilution and anti-rat-HRP.

Immunohistochemistry: Use at approximately
1:20-50 on acetone fixed frozen sections,
followed by incubation with rabbit anti-rat IgG
and an APAAP complex. Unreactive on
standard formaldehyde-fixed, paraffinembedded
material.

Flow cytometry: Optimal conditions still to be
defined.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.