Synonyms

Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF

Uniprot No.

P01375

Source

E.coli-derived human TNF-α/TNFSF2 protein, Val77-Leu233, with C-terminal His tag.

Molecular Weight

Approximately 18.5 kDa.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 0.01 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS.

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.