人亨廷顿相互作用蛋白1相关蛋白(HIP1R)多克隆抗体
Polyclonal Antibody to Huntingtin Interacting Protein 1 Related Protein (HIP1R)
[PROPERTIES]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
[ IMMUNOGEN ]
Immunogen: Recombinant HIP12 (Ser771~Ala1012) expressed in E.coli. 
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
[ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant HIP12 disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3.
[STORAGE AND STABILITY] 
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[IDENTIFICATION]

Figure 1. Western Blot
A. Sample: Recombinant HIP12, Human B. Lane1: Porcine Kidney Tissue Lane2: Human HeLa Cells Lane3: Porcine Brain Tissue Primary Ab: 3µg/mL Rabbit Anti-Human HIP12 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。