兔白介素7(IL7)多克隆抗体

Polyclonal Antibody to Interleukin 7 (IL7)

[ PRODUCT INFORMATION ]

Immunogen: IL7, Rabbit

Clonality: Polyclonal

Host: Cavia

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200µg/mL

UOM: 100µg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant IL7 (Asn26~Asn159) with two N-terminal Tags,His-tag and T7-tag expressed in E.coli.

[ ANTIBODY SPECIFITY ]

The antibody is a cavia polyclonal antibody raised against IL7. It has been selectedfo r it s ab ilit y to re cog ni ze IL7 in immuno hi st o chem i cal st aining an dwestern blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant IL7 (Asn26~Asn159)disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%

[ STORAGE ] 

Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

[ IMAGES ]