I. General information

Synonyms:       MONO-MAC-6

Background:    established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene; more information on this cell line on the following website: "www.monocytes.de"; sister cell line of MONO-MAC-1 (DSM ACC 252); cells are used for the detection of pyrogens

Species:           human (Homo sapiens)

Tissue: N/A

Disease:          acute monocytic leukemia

Gender:           N/A

Morphology:    single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells

Growth Mode: N/A

Doubling Time:           ca. 50-60 hours

DNA Profile:    N/A

Cobioer’s Cell Line Authentication Service

Culture Medium:         RPMI-1640+10%FBS+0.1mM NEAA+ 1 mM sodium pyruvate + 10 μg/ml insulin

We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:     90%FBS+10%DMSO

Mycoplasma:  negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:   CD3 -, CD4 -, CD13 +, CD14 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; note that CD14 expression depends on cultivation conditions; CD14 positivity cannot be guaranteed; image