Synonyms :    B7-H2; ICOS Ligand; B7-like Protein Gl50; B7RP-1  
Host :  CHO cells 
Sequence :  The extracellular domain of mouse CD275 [B7-H2] (NP_056605.1)(Thr48-Lys279) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a. 
Molecular Mass :   56 KDa (monomer) 
Non-lytic :  Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). 
Purity :  ≥98%，by SDS-PAGE under reducing conditions.  
Endotoxin Content :  <0.06 EU/μg as determined by LAL test. 

Activity :  Measured by its binding ability in a functional ELISA. Immobilized human ICOS at 1μg/ml (100μl/well) can bind biotinylated B7-H2(mouse):Fc (mouse) (rec.)(non-lytic) with a linear range of 0.1-1.0μg/ml. Optimal dilutions should be determined byeach laboratory for each application. 
Formulation :  Lyophilized from 0.2μm-filtered solution in PBS. 
Reconstitution :  Reconstitute at 100μg/ml in sterile PBS. 
Use / Stability :  
Stable for at least 1 year after receipt when stored at -20℃. Working aliquots are stable for up to 3 months when stored at -20℃. 
Avoid freeze/thaw cycles.