• 特异性ab195847 detects endogenous levels of Caspase-9 only when phosphorylated at Threonine 125.
• 经测试应用WBmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide corresponding to Human Caspase-9 (phospho T125). A synthesized peptide derived from Human Caspase-9 around the phosphorylation site of Threonine 125.
  Database link: P55211

• 阳性对照
  • TNF treated HeLa whole cell lysates.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.4
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
• 浓度100 µg 浓度为 1 mg/ml 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Cell Biology
   
  • Apoptosis
   
  • Intracellular
   
  • Caspases etc
   
  • Caspases

  • Cancer
   
  • Invasion/microenvironment
   
  • Apoptosis
   
  • Caspases

  • Cancer
   
  • Invasion/microenvironment
   
  • Apoptosis
   
  • Cytochrome C

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolism processes
   
  • Apoptosis

  • Cancer
   
  • Cell Death
   
  • Apoptosis
   
  • Apoptotic Markers
   
  • Caspases

  • Cancer
   
  • Cell Death
   
  • Apoptosis
   
  • Apoptotic Markers
   
  • Cytochrome C

  • Cancer
   
  • Cell Death
   
  • Apoptosis
   
  • Metabolism

应用

靶标

• 功能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
  Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
• 组织特异性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
• 序列相似性Belongs to the peptidase C14A family.
  Contains 1 CARD domain.
• 发展阶段Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
• 翻译后修饰Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

• Anti-Caspase-9 (phospho T125) antibody 图像

  • 

    Western blot - Anti-Caspase-9 (phospho T125) antibody (ab195847)
    All lanes : Anti-Caspase-9 (phospho T125) antibody (ab195847) at 1/500 dilution
    
    Lane 1 : TNF treated HeLa whole cell lysates with antigen-specific peptide
    Lane 2 : TNF treated HeLa whole cell lysates
    
    Lysates/proteins at 12.5 µg per lane.
    
    
    Predicted band size : 46 kDa