HISTRAP FF CRUDE

商家询价

产品名称： HISTRAP FF CRUDE

英文名称： HISTRAP FF CRUDE

产品编号： 11-0004-58

产品价格： 0

产品产地：美国

品牌商标： GE

更新时间： null

使用范围： null

上海基星生物科技有限公司

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地址 : 上海市宝山区友谊路1506号东智广场1109室
邮编 : 201900
所在区域 : 上海
电话 : 138****5983 点击查看
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邮箱 : 342786402@qq.com

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产品详情

11-0003-29	HITRAP DESALTING 100 X 5 ML
11-0004-58	HISTRAP FF CRUDE 5X1 ML
11-0004-59	HISTRAP FF CRUDE 100X1 ML
11-0004-64	STOP PLUG FEMALE, 1/16''
11-0013-02	HITRAP CAPTO Q, 5X1ML
11-0013-03	HITRAP CAPTO Q, 5X5ML
11-0032-73	HITRAP CAPTO MMC, 5X1ML
11-0032-75	HITRAP CAPTO MMC, 5X5ML
11-0034-93	HITRAP MABSELECT SURE, 5X1ML
11-0034-94	HITRAP MABSELECT SURE, 1X5ML
11-0034-95	HITRAP MABSELECT SURE, 5X5ML
17-0402-01	HITRAP PROTEIN A HP, 5 X 1 ML
17-0402-03	HITRAP PROTEIN A HP,2 X 1 ML
17-0403-01	HITRAP PROTEIN A HP,1 X 5 ML
17-0403-03	HITRAP PROTEIN A HP, 5 X 5 ML
17-0404-01	HITRAP PROTEIN G HP, 5 X 1 ML
17-0404-03	HITRAP PROTEIN G HP, 2 X 1 ML
17-0405-01	HITRAP PROTEIN G HP,1 X 5 ML
17-0405-03	HITRAP PROTEIN G HP, 5 X 5 ML
17-0406-01	HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01	HITRAP HEPARIN HP,1 X 5 ML
17-0407-03	HiTrap Heparin HP 5x5ml

HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates

Optimized for purification of histidine-tagged* proteins directly from homogenized, unclarified cell lysates.
Simply homogenize and load your sample directly.
High binding capacity, approx. 40 mg/ml medium and negligible Ni²⁺ leakage.
Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
Convenient kit provides columns and buffers for quick and easy purifications.
Simple operation with a syringe, pump, or chromatographic system such as ÄKTAdesign.

HisTrap™ FF crude Columns/HisTrap FF crude Kit - Convenient Purification from Unclarified Lysates

Technical Information

* For licensing information, see Legal Info.

Customer feedback on Ni Sepharose 6 Fast Flow.

TECHNICAL SPECIFICATIONS
Medium	Ni Sepharose 6 Fast Flow
Column volume	1 ml and 5 ml
Column dimensions	0.7 × 2.5 cm (1 ml) 1.6 × 2.5 cm (5 ml)
Dynamic binding capacity*	Approx. 40-mg histidine-tagged protein/ml medium
Recommended flow rate^†	1 ml/min (1 ml) 5 ml/min (5 ml)
Max. pressure^†	3 bar (0.3 MPa, 42 psi)
Compatibility during use	Stable in all commonly used buffers, reducing agents, denaturing agents and detergents, see Ni Sepharose™ High Performance - High-performance Purification
Chemical stability^‡	For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS
pH stability^‡	2-14 (short term), 3-12 (long term)
Storage	20% ethanol
Storage temperature	+4°C to 30°C
* Protein-dependent † H₂O at room temperature ‡ Ni²⁺-stripped medium

HisTrap™ FF crude columns allow simple, one-step purification of histidine-tagged proteins without sample pretreatment such as centrifugation and filtration.

HisTrap™ FF crude 1-ml and 5-ml columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates. Direct loading of unclarified sample both saves time and prevents degradation of sensitive target protein.

HisTrap™ FF crude columns are prepacked with Ni Sepharose™ 6 Fast Flow. For more information see Ni Sepharose™ 6 Fast Flow - Scale-up Purification. For column technical specifications, see HisTrap™ FF Columns - Easy Scale-up Purification (HisTrap™ FF Columns). For best results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, e.g. by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.

HisTrap FF crude Kit provides convenient purification of histidine-tagged proteins. The kit contains three HisTrap FF crude 1-ml columns, phosphate buffer, pH 7.4 (2 × 50 ml [8× concentrate]), 2 M imidazole, pH 7.4, all necessary connectors, a syringe, and instructions.

Four repeated purifications of MBP-(His)₆ from E. coli extract on HisTrap FF crude 1 ml. The medium was subjected to Ni²⁺-ion stripping, cleaning-in-place, and recharging between runs.

HisTrap FF crude columns save time in the purification of histidine-tagged proteins compared to conventional IMAC.