GSTRAP FF-色谱柱 -耗材-生物在线
上海基星生物科技有限公司
GSTRAP FF

GSTRAP FF

商家询价

产品名称: GSTRAP FF

英文名称: GSTRAP FF

产品编号: 17-5130-01

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
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17-5130-01 GSTRAP FF, 5X1ML
17-5130-02 GSTRAP FF, 2X1ML
17-5130-05 GSTRAP FF, 100 X 1 ML
17-5131-01 GSTRAP FF, 1X5ML
17-5131-02 GSTRAP FF 5 X 5 ML
17-5131-05 GSTRAP FF, 100 X 5 ML
17-5143-01 HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02 HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01 HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01 HITRAP DEAE FF 5 X 5 ML
17-5155-01 HITRAP CM FF, 5 X 5 ML
17-5156-01 HITRAP Q FF, 5 X 5 ML
17-5157-01 HITRAP SP FF, 5 X 5 ML
17-5158-01 HITRAP Q XL, 5 X 1 ML
17-5159-01 HITRAP Q XL, 5 X 5 ML
17-5160-01 HITRAP SP XL, 5 X 1 ML
17-5161-01 HITRAP SP XL, 5 X 5 ML
17-5162-01 HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01 HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01 HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01 HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01 HITRAP PHENYL HP, 5 X 5 ML
17-5196-01 HITRAP OCTYL FF, 5 X 5 ML
17-5197-01 HITRAP BUTYL FF, 5 X 5 ML
17-5247-01 HISTRAP HP 5 X 1 ML
17-5247-05 HISTRAP HP 100 X 1 ML
17-5248-01 HISTRAP HP 1 X 5 ML
17-5248-02 HISTRAP HP 5 X 5 ML
17-5248-05 HISTRAP HP 100 X 5 ML
17-5255-01 HISTRAP FF 5 X 5 ML
17-5255-02 HISTRAP FF 100 X 5 ML
17-5281-01 GSTRAP HP 5 X 1 ML
17-5281-05 GSTRAP HP 100 X 1 ML
17-5282-01 GSTRAP HP 1 X 5 ML
17-5282-02 GSTRAP HP 5 X 5 ML
17-5282-05 GSTRAP HP, 100 X 5 ML
17-5286-01 HISTRAP FF CRUDE 5X5 ML
17-5286-02 HISTRAP FF CRUDE 100X5 ML
17-5319-01 HISTRAP FF 5 X 1 ML
17-5319-02 HISTRAP FF 100 X 1 ML
17-5441-22 HITRAP CAPTO S, 5X1 ML
17-5441-23 HITRAP CAPTO S, 5X5 ML
17-5468-51 HiTrap Capto SP ImpRes 5x1mL
17-5468-55 HiTrap Capto SP ImpRes 5x5mL
17-5470-51 HiTrap Capto Q ImpRes 5x1mL
17-5470-55 HiTrap Capto Q ImpRes 5x5mL
17-6002-33 HITRAP IEX SELECTION KIT
18-1142-75 REC PROTEIN PURIF HANDBOOK
28-4010-81 HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77 HISTRAP FF CRUDE KIT
28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP, 5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit, 7x1
28-9075-46 StrepTrap HP 5 x 1mL
28-9075-47 StrepTrap HP 1 x 5mL
28-9075-48 StrepTrap HP 5 x 5mL
28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml

GSTrap™ FF Columns - Easy Scale-up Purification

  • Fast and simple one-step purification of GST-tagged* proteins.
  • Sepharose™ 4 Fast Flow provides good flow properties.
  • Binds approx. 10 mg of recombinant GST/ml medium.
  • Easy scale-up by connecting the columns in series.
  • Simple operation with a syringe, pump, or chromatography system such as ÄKTA™design.

GSTrap™ FF Columns - Easy Scale-up Purification

Technical Information


Click here to view this image in high resolution
GSTrap™ FF columns are 1-ml and 5-ml HiTrap™ columns prepacked with Glutathione Sepharose™ 4 Fast Flow

 

GSTrap™ FF columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.

 

For an introduction to Glutathione S-transferase (GST*) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System.

 

Click here to view this image in high resolution
SDS-PAGE on ExcelGel™ SDS Gradient 8-18% using Multiphor™ II followed by silver staining.

 

Click here to view this image in high resolution
Purification of GST-tagged protein on GSTrap™ FF 5 ml.

 

TECHNICAL SPECIFICATIONS
Medium  Glutathione Sepharose™ 4 Fast Flow 
Column volume  1 ml and 5 ml 
Column dimensions  0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml) 
Recommended flow rate*  0.2-2 ml/min (1 ml); 1-10 ml/min (5 ml) 
Max. pressure*  3 bar (0.3 MPa, 42 psi)  
pH stability  3-12 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
* H2O at room temperature.
Binding of GST to glutathione is flow-rate dependent and lower flow rates often
increase the binding capacity. This is important to consider, especially during
sample loading and elution.

http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=163506