pBluescript II SK(-)
产品名称： pBluescript II SK(-)
英文名称： pBluescript II SK(-)
产品编号： Biovector-01128569
产品价格： 0
产品产地： Biovector NTCC Inc. USA
品牌商标： Biovector,NTCC, Addgene, ATCC,
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
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- 邮箱 : Biovector@163.com
|
Order ID |
Name |
Description |
|
Biovector-01128569 |
pBluescript II SK(-) |
pBluescript II SK(-),DNA.15uL,AmpR.Storage:-20℃ |
Transformation of plasmid DNA to competent E. Coli cells
- Thaw competent cells on ice. 20–200µL per tube
- Add max. 1-3µL of plasmid
- Mix very gently!
- Incubate the tubes on ice for 30 min
- Heat shock the cells for 45 sec to 2 min at 42°C
- Place the tubes immediately on ice for at least 2 min
- Add 800µL of SOC medium to each tube
- Incubate for 1 hour at 37°C and shake vigorously
- Spin down briefly and remove most supernatants
- Resuspend cell pellet with the rest SOC medium in the tube by pipetting
- Plate out the suspension on a LB agar plate containing the appropriate antibiotic. Incubate the plates overnight at 37°C
LOCUS pBluescript II SK(-) 2959 bp DNA CIRCULAR SYN 19-MAR-2009
DEFINITION
ACCESSION pBluescript II SK(-)
KEYWORDS .
SOURCE Unknown.
ORGANISM Unknown
Unclassified.
REFERENCE 1 (bases 1 to 2959)
AUTHORS Self
JOURNAL Unpublished.
COMMENT SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES Location/Qualifiers
CDS 21..327
/gene="f1(-)origin"
misc_feature 600..643
/gene="T7/M13F"
CDS complement (461..615)
/gene="lacZ"
misc_feature complement (770..826)
/gene="T3/M13R"
misc_feature 1156..1823
/gene="pUC origin"
CDS complement (1974..2831)
/gene="Amp"
BASE COUNT 707 a 755 c 731 g 766 t
ORIGIN
1 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
61 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
121 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
181 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
241 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
301 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT
361 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
421 TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC
481 GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG
541 GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT
601 GTAAAACGAC GGCCAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC