犬表皮生长因子(EGF)多克隆抗体

Polyclonal Antibody to Epidermal Growth Factor (EGF)

[ PRODUCT INFORMATION ]

Immunogen: EGF, Canine

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200µg/mL

UOM: 100µg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant EGF (His367~Phe489) expressed in E.coli.

Sequence: The target protein is fused with N-terminal His-Tag and its sequenceis listed below.

MGHHHHHHSG SEF- HGCT LGCENIPGSY YCTCPVGFIL LPDGKRCHQL IACPSNTSKC

S H D C V LT S D G PI C F CP E G S V LE A D G K T C S G CS S P D N G G CS Q L C L P L S P V S

WECGCFPGYD LQLDKQSCAA SGPQPFLLF

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against EGF. It has beenselected for its ability to recognize EGF in immunohistochemical staining andwestern blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ] 

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.

[ QUALITY CONTROL ] 

Content:The quality control contains recombinant EGF (His367~Phe489)disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%

[ STORAGE ] 

Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。