• 经测试应用IHC-Pmore details
• 种属反应性
  与反应: Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD16 aa 200 to the C-terminus. The exact sequence is proprietary.
  Database link: P08637

• 阳性对照
  • Human liver and thymus, Rat liver tissues.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
• 浓度100 µl 浓度为 0.354 mg/ml 
  
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR16784
• 同种型IgG
• 研究领域
  • Immunology
   
  • Cell Type Markers
   
  • CD
   
  • NK Cells

  • Stem Cells
   
  • Hematopoietic Progenitors
   
  • Hematopoietic Stem Cells
  • Human Lineage Negative

  • Stem Cells
   
  • Hematopoietic Progenitors
   
  • Lymphoid
   
  • NK Cell Lineage

  • Stem Cells
   
  • Hematopoietic Progenitors
   
  • Myeloid
   
  • Monocytic Lineage

  • Stem Cells
   
  • Hematopoietic Progenitors
   
  • Myeloid
   
  • Neutrophil Lineage

应用

靶标

• 功能Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
• 组织特异性Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
• 序列相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
• 翻译后修饰Glycosylated. Contains high mannose- and complex-type oligosaccharides.
  The soluble form is produced by a proteolytic cleavage.
• 细胞定位Cell membrane. Secreted. Exists also as a soluble receptor.

• Anti-CD16 antibody [EPR16784] - C-terminal 图像

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of rat liver tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophage of Human thymus tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - C-terminal (ab198507)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of Human liver tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.