Orexin Prepro,食欲素前体蛋白OX抗体

产品编号HZ-11520R
英文名称Orexin Prepro
中文名称食欲素前体蛋白OX抗体
别 名HCRT; Hypocretin; OX; PPORX; PPOX; Prepro orexin; OREX_HUMAN; Orexin; Hcrt; Hypocretin-1; Hcrt1; Orexin-B; Hypocretin-2; Hcrt2.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 神经生物学 内分泌病
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
Orexin Prepro,食欲素前体蛋白OX抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量3kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Orexin Prepro (70-97aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Orexin Prepro,食欲素前体蛋白OX抗体PubMedPubMed
产品介绍background:
Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.

Function:
Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.

Subcellular Location:
Cytoplasmic and Endoplasmic reticulum

Tissue Specificity:
Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Post-translational modifications:
Specific enzymatic cleavages at paired basic residues yield the different active peptides.

Orexin Prepro,食欲素前体蛋白OX抗体DISEASE:
Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1) [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Note=Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients.

Similarity:
Belongs to the orexin family.

Gene ID:
3060

Database links:
Entrez Gene: 3060 Human
Omim: 602358 Human
SwissProt: O43612 Human
Unigene: 158348 Human

Orexin Prepro,食欲素前体蛋白OX抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.