TBR2,脱中胚蛋白抗体

产品编号HZ-11331R
英文名称TBR2
中文名称脱中胚蛋白抗体
别 名Eomes; EOMES_HUMAN; Eomesodermin; Eomesodermin homolog; t box brain 2; T-box brain protein 2; T-brain-2; Tbr 2; TBR-2.
说 明 书0.1ml 0.2ml
研究领域细胞生物 发育生物学 神经生物学 干细胞 细胞类型标志物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Cow, Horse, Sheep,
TBR2,脱中胚蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量73kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TBR2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TBR2,脱中胚蛋白抗体PubMedPubMed
产品介绍background:
EOMES (eomesodermin homolog), also known as TBR2 (T-box-brain2), is the 686 amino acid human homolog of the mouse Eomes protein that contains one T-box DNA binding domain. Genes that contain T-box domains encode proteins that function as transcription factors and are often involved in the regulation of various developmental events. Localized to the nucleus and expressed in the developing brain, EOMES is thought to be involved in neuronal migration and division and may play a role in trophoblast development and gastrulation. Silencing of the EOMES gene can cause mutated or arrested development and may lead to microcephaly disorders which are characterized by reduced head circumference and a malformed brain.

Function:
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in CD8+ T-cells.

DISEASE:
Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.

Similarity:
Contains 1 T-box DNA-binding domain.

TBR2,脱中胚蛋白抗体Database links:
Entrez Gene: 8320 Human
Entrez Gene: 13813 Mouse
Omim: 604615 Human
SwissProt: O95936 Human
SwissProt: O54839 Mouse
Unigene: 591663 Human
Unigene: 200692 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TBR2 Polyclonal Antibody, Unconjugated(HZ-11331R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining