Product: Anti-mSmac pAb
Cat. No.: PC-544 (100 μg)
Synonym:
Anti-DIABLO
Background:
The inhibitor of apoptosis proteins (IAPs)
regulate programmed cell death by inhibiting
members of the caspase family of enzymes. A
novel mammalian protein that binds to IAPs and
neutralizes the inhibitory effect of IAPs on
caspases was recently identified and designated
Smac/DIABLO. Smac/DIABLO is a mitochondrial
protein that is released along with
cytochrome c during apoptosis and activates
cytochrome c/Apaf-1/capase-9 pathway.
Analysis of the structural basis of Smac/DIABLO
reveals that the N-terminal amino acids are
required for binding of Smac/DIABLO to IAPs
and activation of caspases. Smac/DIABLO is
expressed in a variety of human and mouse
tissues.
Specificity:
Recognizes mSmac.
Positive Control:
Mouse heart tissue lysate (~ 25 kDa band can
be detected).
Species Reactivity:
Human, mouse and rat. Other species not tested.
Host:
Rabbit
Immunogen:
Raised against a peptide (SDEGADQEEEAYL
RED) corresponding to amino acids 222 to 237
of mouse Smac/DIABLO.
Format:
100 μg of polyclonal antibody in PBS containing
0.02 sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 - 1 μg/mL.

Immunohistochemistry: Use at ~ 2 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of Smac in mouse
heart tissue lysate in the absence (A) or presence (B)
of blocking peptide and in rat heart tissue lysate (C)
with anti-mSmac pAb at 1 μg/mL.
Figure 2: Immunohistochemistry staining of mouse
spleen tissue using anti-mSmac pAb at 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.