• 形式Liquid
• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 纯度Tissue culture supernatant
• 克隆单克隆
• 克隆编号EP2460
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Alzheimer's disease
   
  • Other

  • Neuroscience
   
  • Cell Adhesion Proteins
   
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
   
  • Cell Type Marker
   
  • Neuron marker
   
  • Soma marker

  • Signal Transduction
   
  • Cytoskeleton / ECM
   
  • Cytoskeleton
  • Intermediate Filaments
   
  • Class IV
   
  • Neurofilaments

  • Stem Cells
   
  • Lineage Markers
   
  • Ectoderm

  • Developmental Biology
   
  • Lineage specification
   
  • Ectoderm

应用

• 应用说明ICC/IF: 1/100 - 1/250. 
  IHC-P: 1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. 
  IP: 1/10. 
  WB: 1/100000 - 1/500000. Predicted molecular weight: 102 kDa. 
  
  Is unsuitable for Flow Cyt. 
  
  
  Not yet tested in other applications. 
  Optimal dilutions/concentrations should be determined by the end user.

靶标

• 相关性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
• 细胞定位Cytoplasm
• 数据库链接
  • Entrez Gene: 4741 Human
  • Omim: 162250 Human
  • SwissProt: P07197 Human
  • Unigene: 458657 Human
• 别名
  • 150kDa medium antibody
  • NEF3 antibody
  • NEFM antibody

  • Neurofilament 3 antibody
  • NF160 antibody
  • NFM antibody

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Anti-160 kD Neurofilament Medium antibody [EP2460] 图像

• 

  Western blot - 160 kD Neurofilament Medium antibody [EP2460] (ab92539)
  All lanes : Anti-160 kD Neurofilament Medium antibody [EP2460] (ab92539) at 1/100000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : SH-SY5Y cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
  
  Predicted band size : 102 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 160 kD Neurofilament Medium antibody [EP2460] (ab92539)
  ab92539, at 1/250 dilution, staining 160 kD Neurofilament Medium in formalin-fixed, paraffin-embedded Human normal brain tissue by immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 160 kD Neurofilament Medium antibody [EP2460] (ab92539)
  ab92539, at 1/250 dilution, staining 160 kD Neurofilament Medium in formalin-fixed, paraffin-embedded Human glioma tissue by immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - 160 kD Neurofilament Medium antibody [EP2460] (ab92539)
  TUNEology 波长可调检测卡盒-->