GRID2IP,谷氨酸受体δ2/GluR-δ2抗体

产品编号HZ-11347R
英文名称GRID2IP
中文名称谷氨酸受体δ2/GluR-δ2抗体
别 名Delphilin; delta 2 interacting protein 1; Grid Delta2; GluR-Delta 2 Glutamate receptor; GRD2I_HUMAN; grid2ip; ionotropic.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
GRID2IP,谷氨酸受体δ2/GluR-δ2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量132kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human GRID2IP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
GRID2IP,谷氨酸受体δ2/GluR-δ2抗体PubMedPubMed
产品介绍background:
Grid2ip is a postsynaptic scaffolding protein that contains one formin homology 2 (FH2) domain and two PDZ (postsynaptic density-95/discs-large/ZO-1) domains. Expressed in Purkinje cells of the cerebellum and localizing specifically to parallel fiber synapses, Grid2ip interacts with the C-terminus of GluR-Delta 2 and, via this interaction, links GluR-Delta 2 with various signaling molecules and the Actin cytoskeleton. GluR-Delta 2 is a glutamate receptor with an important role in motor learning, cerebellar wiring and synaptic plasticity. Due to alternative splicing events, three Grid2ip isoforms exist, namely L-delphilin, S-delphilin (or delphilin-Alpha) and delphilin-Beta. Each isoform exhibits individual expression patterns and protein interactions. Isoform 2, delphilin-Alpha, is palmytoylated, a modification that is essential for the enhanced expression of GluR-Delta 2 on the cell surface. This modification of delphilin-Alpha also mediates the accumulation of delphilin-Alpha in dendritic spines.

Function:
Postsynaptic scaffolding protein at the parallel fiber-Purkinje cell synapse, where it may serve to link GRID2 with actin cytoskeleton and various signaling molecules.

Subcellular Location:
Cell junction > synapse > postsynaptic cell membrane.

GRID2IP,谷氨酸受体δ2/GluR-δ2抗体Similarity:
Contains 1 FH2 (formin homology 2) domain.
Contains 2 PDZ (DHR) domains.

Gene ID:
392862

Database links:
Entrez Gene: 392862 Human
Omim: 610639 Human
SwissProt: A4D2P6 Human
Unigene: 512174 Human

GRID2IP,谷氨酸受体δ2/GluR-δ2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-GRID2IP Polyclonal Antibody, Unconjugated(HZ-11347R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-GRID2IP Polyclonal Antibody, Unconjugated(HZ-11347R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-DRID2IP Polyclonal Antibody, Unconjugated(HZ-11347R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.