Synonyms :  V-set Domain-containing T Cell Activation Inhibitor 1; VTCN1; Immune Costimulatory Protein B7-H4; T Cell Costimulatory Molecule B7x  
Host :  CHO cell 
Sequence :  The extracellular domain of human B7-H4(NP_078902.2)(Phe29-Ala258) is fused to the N-terminus of the Fc region of mouse IgG2a. 
Molecular Mass :  55 KDa (monomer) 
Purity :  >98%，by SDS-PAGE under reducing conditions.  
Endotoxin Content :  <0.06 EU/μg as determined by LAL test. 

Activity ：  Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well place coated with 500 ng/ml anti-CD3 and 10 ug/ml human B7H4/Fc.The presence of human B7H4/Fc at 10 ug/ml inhibited anti CD3 response by 30-50%. Optimal dilutions should be determined by each laboratory for each application. 
Formulation :  Lyophilized from 0.2μm-filtered solution in PBS. 
Reconstitution :  Reconstitute at 100μg/ml in sterile PBS. 
Use / Stability :  
Stable for at least 1 year after receipt when stored at -20℃. Working aliquots are stable for up to 3 months when stored at -20℃. 
Avoid freeze/thaw cycles.