钙黄绿素 AM 是一种膜渗透性荧光酯酶底物，通常用于检测细胞活性和质膜完整性。作为一种近中性的底物，钙黄绿素 AM 可以自由、无创地扩散穿过质膜并渗透到活细胞的胞质溶胶中。一旦进入细胞内，代谢活跃细胞中的胞质酯酶会迅速将非荧光钙黄绿素 AM 底物水解成钙黄绿素产物，并由具有完整质膜的细胞保留。由于死细胞缺乏酯酶活性，因此仅标记活细胞。在蓝光激发下，钙黄绿素发出强烈的绿色荧光，与细胞酯酶的活性和样品中存在的活细胞数量成正比。与其他活细胞标记试剂（如 BCECF AM 或羧基荧光素二乙酸酯）相比，钙黄绿素 AM 荧光更亮、光稳定性更好，低细胞毒性有利于细胞追踪、细胞凋亡和细胞毒性的研究。
AAT Bioquest 提供多种可供选择的用于细胞活力分析的钙黄绿素 AM 产品。除了传统的绿色荧光钙黄绿素 AM 之外，我们还提供发出蓝色、橙色、红色和深红色荧光的钙黄绿素 AM 衍生物。
钙黄绿素活性染料选择指南
|
Dye |
Calcein Blue |
CytoCalcein™ Violet 450 |
Calcein UltraBlue™ |
CytoCalcein™ Violet 500 |
Calcein UltraGreen™ |
Calcein |
Calcein Orange™ |
Calcein Red™ |
Calcein Deep Red™ |
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|
Principle |
In live cells, non-fluorescent calcein AM is converted to a fluorescent calcein product after AM ester hydrolysis by intracellular esterases. |
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Readout |
Live cells are highly fluorescent and easily distinguishable from dead cells, which are non-fluorescent. |
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|
Laser(nm) |
UV (350) |
Violet (405) |
UV (350) |
Violet (405) |
Blue (488) |
Blue (488) |
Green (532) |
Yellow (561-568) |
Red (633-647) |
|
|
Ex/Em(nm) |
354/441 |
406/445 |
359/458 |
420/505 |
492/514 |
501/521 |
531/545 |
562/576 |
643/663 |
|
|
Multiplexable |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
|
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Dye Compatibility |
Compatible with green and red fluorescent proteins, indicators, and other probes |
Compatible with green and red fluorescent proteins, indicators, and other probes |
Compatible with green and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and red fluorescent proteins, indicators, and other probes |
Compatible with blue and green fluorescent proteins, indicators, and other probes |
Compatible with blue and green fluorescent proteins, indicators, and other probes |
|
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Sample Type |
Live Cells |
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Platform |
Fluorescence Microscope, Fluorescence Microplate Reader, Flow Cytometer |
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Cat No. |
AM Ester |
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